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KMID : 0616120190500040299
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Korean Journal of Pharmacognosy
2019 Volume.50 No. 4 p.299 ~ p.304
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Antioxidative-activity of Phellinus baumii Pilat in Caenorhabditis elegans
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Kim Jun-Hyeong
Kwon Kang-Mu
Yang Jae-Heon
Ki Byeol-Hui
Kim Dae-Keun
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Abstract
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In order to research the anti-oxidative activity of methanol extract of Phellinus baumii Pilat (Hymenochaetaceae), Caenorhabditis elegans model system was used. Ethyl acetate soluble fraction of the methanol extract showed the most potent DPPH radical scavenging activity. The ethyl acetate fraction was measured on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance with reactive oxygen species (ROS) level in C. elegans. Furthermore, to see if regulation of stress-response gene is responsible for the increased stress tolerance of C. elegans which treated by the ethyl acetate fraction, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of P. baumii increased SOD and the catalase activities in a dose?dependent manner in C. elegans, reduced ROS accumulation dose-dependently. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the control worms.
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KEYWORD
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Phellinus baumii Pilat, Caenorhabditis elegans, Anti-oxidative activity, Catalase, SOD
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